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Thus, to enhance teachers’ prosocial impact, more opportunities to realise their collaboration should be considered. Results indicate that teacher collaboration contributes to less teacher turnover intention via prosociality. Future studies should incorporate alternative designs. Limitations of this study include cross-sectional data that may limit the potential for causal inferences, and self-report data. Second, results supported partial mediating roles of prosocial impact and prosocial motivation between teacher collaboration and turnover intention. The results, first, supported the hypotheses: the high perception of teacher collaboration in school predicted high perceived prosocial impact high perceived prosocial impact predicted high perceived prosocial motivation and high perceived prosocial motivation predicted decreased turnover intention. A structural equational model was employed to examine the mediating roles of prosocial impact and prosocial motivation in the relationships between teacher collaboration and their turnover intention. This study was conducted through a cross-sectional survey of 260 elementary and junior high school teachers in Japan. Prosociality was measured as prosocial impact and prosocial motivation. The purpose of this paper is to investigate the mediating role of prosociality, which is defined in terms of helping and benefitting others, between teacher collaboration and their turnover intentions. Implications for research on burnout, job satisfaction, positive organizational scholarship and job design are discussed. The results suggest that perceptions of benefiting others may protect service employees against the decreased job satisfaction and increased burnout typically associated with perceptions of harming others. In Study 2, a survey of 79 school teachers, perceived prosocial impact moderated the association between perceived antisocial impact and burnout, and this moderated relationship was mediated by moral justification the results held after controlling for common antecedents of burnout. In Study 1, a survey of 377 transportation service employees and 99 secretaries, perceived prosocial impact moderated the negative association between perceived antisocial impact and job satisfaction, such that the association decreased as perceived prosocial impact increased. We conducted two studies to test the hypothesis that perceptions of benefiting others attenuate the detrimental effects of perceptions of harming others on the well-being of service employees. Service employees often perceive their actions as harming and benefiting others, and these perceptions have significant consequences for their own well-being.